| dc.description.abstract | The study was conducted in the Microbiology Laboratory of the Department of
Microbiology and Hygiene, Sylhet Agricultural University (SAU) during the period
of July 2011 to June 2012.
Fresh samples of different stages of guava leaves such as immature, mature and adult
(Pisidium guavaja) were collected from in and around Sylhet Agricultural University
(SAU Campus), Government Goat farm, Balochar, Shivgang and Tilagor, Sylhet.
Randomly leaf samples were collected into plastic zip lock bags with appropriate
labeling and stored in an ice cooler until being transported to the laboratory for
extraction to find the antibacterial sensitivity of different stages guava leaf extract
against Escherichia coli isolated from commercial broilers. Isolation and
identification of bacteria were confirmed on the basis of their morphology, staining,
cultural and biochemical properties. Antibiotic sensitivity test was studied by Disc
diffusion method.
In the present research work the mature guava leaves extract showed better, adult
guava leaves extracts medium and immature extracts poor antibacterial activity
against
Escherichia .coli. All the extracts showed varying degrees of antimicrobial activity on
the microorganism tested resulting in zone of inhibition of 7.0– 9.0mm. | en_US |
