| dc.description.abstract | Studies were conducted to investigate current status of antibiotics used in aquaculture and
oxytetracycline’s effects on Sarpunti (Puntius sarana) muscle in Golapgonj and Jointapur
upazilla of Sylhet. Additional investigations also were done to know microbial load and other
water quality parameters of the same area. A questionnaire survey was conducted with various
stakeholders engaged with aquaculture of study area from June to August, 2013. The study
showed that fish farmers used various antibiotics mainly Renamycin, Aquamysine, Oxy-DoxF,
Oxytensin, Amoxyfish, Amoxivet, Tetravet, Orgamysin, Cottrim vet and their generic
names were oxytetracycline, chlorotetracycline, amoxicillin, co-trimoxazole in aquaculture
for fish health management and disease treatment. Oxytetracycline (OTC), the most widely
used antibiotic, was fed to Sarpunti (Puntius sarana) at the rate of 4 g/kg through fish diet, and
changes in water quality parameters and bacterial content were determined for a period of 21
days. Pond water, sediment and fish muscle were tested before antibiotic treatment and after
oxytetracycline medicated feed. The initial oxytetracycline accumulation was 5070.0 ppb
which reduced considerably to 1190.0 ppb after 5 days of stopping oxytetracycline treated
diets and this antibiotic was not detected after 10 days. Physico-chemical parameters of pond
water were determined. Before antibiotic treatment , temperature, dissolve oxygen (DO), pH
and total hardness of sarpunti fish pond was 25.87 ± 0.015°C , 4.516 ± 0.25 mg/l and 7.413 ±
0.09 and total hardness of 822.33 ± 1.52 ppm, respectively which reached a value 27.33 ±
0.015°C, 5.27 ± 0.025 mg/l, 7.773
± 0.04 and 769.66 ± 2.08 ppm, respectively indicating
little change after 21 days. Before antibiotic treatment, bacterial load in sarpunti fish pond was
9.417 ± 0.035×10
cfu/g
in fish gills, 8.52
3
cfu/ml in water, 7.31 ± 0.04×10
7
cfu/g in sediment, 6.51 ± 0.04 ×10
6
cfu/g in fish intestine, respectively which was significantly
reduced to 5.28 ± 0.02×10
± 0.035 ×10
7
3
cfu/ml in water, 2.91 ± 0.02×10
7
in sediment, 2.21 ± 0.02×10
6
cfu/g in gills, 2.39 ± 0.02×10
7
cfu/g in intestine, respectively. During the field observation
some problems were identified while using antibiotics which included poor understanding of
farmers about the mode of action, real application of antibiotics, exact reason of disease, in
adequate with drawl period and some adverse effects on fish and human health. | en_US |
